ez tet plko puro (Addgene inc)
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Addgene inc
ez tet plko puro
Ez Tet Plko Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ez+tet+plko+puro/EZ-Tet-pLKO-Puro+(Plasmid+%2385966)/med_rxiv__64898__2025__12__19__25342655-277-29-30
Average 94 stars, based on 44 article reviews
Ez Tet Plko Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ez+tet+plko+puro/EZ-Tet-pLKO-Puro+(Plasmid+%2385966)/med_rxiv__64898__2025__12__19__25342655-277-29-30
Average 94 stars, based on 44 article reviews
ez tet plko puro - by Bioz Stars,
2026-10
94/100 stars
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Knockdown:Article Title: Augment proteasome inhibitor efficacy activates CD8 + T cell-mediated antitumor immunity in breast cancer Article Snippet: Guide RNAs (gRNAs) were subsequently cloned and inserted into lentiCRISPRv2 Puro (Addgene #52961) or TCLV2 (Addgene #87360) according to the standard protocol. .. For gene knockdown, the corresponding shRNA sequences were subsequently cloned and inserted into pLKO.1-Puro (Addgene #8453) or shRNA:Article Title: Augment proteasome inhibitor efficacy activates CD8 + T cell-mediated antitumor immunity in breast cancer Article Snippet: Guide RNAs (gRNAs) were subsequently cloned and inserted into lentiCRISPRv2 Puro (Addgene #52961) or TCLV2 (Addgene #87360) according to the standard protocol. .. For gene knockdown, the corresponding shRNA sequences were subsequently cloned and inserted into pLKO.1-Puro (Addgene #8453) or Article Title: Colorectal cancer risk variants in the 11q13.4 locus are associated with variable POLD3 transcript expression which may promote DNA damage and telomere shortening in colorectal cancer cells Article Snippet: For the Genomics England CRC cohort (N=163), Spearman correlation coefficients were similarly calculated among POLD3 transcripts within genotype-defined groups (heterozygous vs homozygous carriers of the rs577 risk allele). .. POLD3 shRNA target sequences were obtained from Broad institute GPP web portal (Clone ID: TRCN0000233258, TRCN0000233261) and were inserted into shRNA sequences as described here. shRNAs were cloned into Clone Assay:Article Title: Augment proteasome inhibitor efficacy activates CD8 + T cell-mediated antitumor immunity in breast cancer Article Snippet: Guide RNAs (gRNAs) were subsequently cloned and inserted into lentiCRISPRv2 Puro (Addgene #52961) or TCLV2 (Addgene #87360) according to the standard protocol. .. For gene knockdown, the corresponding shRNA sequences were subsequently cloned and inserted into pLKO.1-Puro (Addgene #8453) or Article Title: Supporting Information for OGA mutant aberrantly hydrolyzes O-GlcNAc modification from PDLIM7 to modulate p53 and cytoskeleton in promoting cancer cell malignancy Article Snippet: To generate pgPDLIM7 for protein purification, OGT and PDLIM7 were subcloned into pETDUET (MilliporeSigma) in which PDLIM7 was fused with an N-terminal His6tag. .. The shRNAs targeting the 3’-UTR of OGA (TRCN0000285410) or PDLIM7 (TRCN0000350814) were selected from the Genetic Perturbation Platform (Broad Institute) and cloned into the Article Title: Colorectal cancer risk variants in the 11q13.4 locus are associated with variable POLD3 transcript expression which may promote DNA damage and telomere shortening in colorectal cancer cells Article Snippet: For the Genomics England CRC cohort (N=163), Spearman correlation coefficients were similarly calculated among POLD3 transcripts within genotype-defined groups (heterozygous vs homozygous carriers of the rs577 risk allele). .. POLD3 shRNA target sequences were obtained from Broad institute GPP web portal (Clone ID: TRCN0000233258, TRCN0000233261) and were inserted into shRNA sequences as described here. shRNAs were cloned into Over Expression:Article Title: HIF1α-PHD1-FOXA1 Axis Orchestrates Hypoxic Reprogramming and Androgen Signaling Suppression in Prostate Cancer. Article Snippet: The HA-tagged HIF1α expression plasmid was obtained from Addgene (plasmid #18949, Watertown, MA, USA), while additional constructs including Myc-tagged HIF1α, V5-tagged PHD1, PHD2, and PHD3, as well as shRNA expression vectors targeting relevant genes, were generously provided by Prof. Johanna Myllyharju (University of Oulu). .. For inducible gene silencing or overexpression, the pLKO.1-TetON-Puro-HIF1α (Addgene #118704, Watertown, MA, USA) and Article Title: HIF1α-PHD1-FOXA1 Axis Orchestrates Hypoxic Reprogramming and Androgen Signaling Suppression in Prostate Cancer Article Snippet: The HA-tagged HIF1α expression plasmid was obtained from Addgene (plasmid #18949, Watertown, MA, USA), while additional constructs including Myc-tagged HIF1α, V5-tagged PHD1, PHD2, and PHD3, as well as shRNA expression vectors targeting relevant genes, were generously provided by Prof. Johanna Myllyharju (University of Oulu). .. For inducible gene silencing or overexpression, the pLKO.1-TetON-Puro-HIF1α (Addgene #118704, Watertown, MA, USA) and Plasmid Preparation:Article Title: PRPF40A induces inclusion of exons in GC-rich regions important for human myeloid cell differentiation Article Snippet: .. Biomarker Discovery:Article Title: Colorectal cancer risk variants in the 11q13.4 locus are associated with variable POLD3 transcript expression which may promote DNA damage and telomere shortening in colorectal cancer cells Article Snippet: For the Genomics England CRC cohort (N=163), Spearman correlation coefficients were similarly calculated among POLD3 transcripts within genotype-defined groups (heterozygous vs homozygous carriers of the rs577 risk allele). .. POLD3 shRNA target sequences were obtained from Broad institute GPP web portal (Clone ID: TRCN0000233258, TRCN0000233261) and were inserted into shRNA sequences as described here. shRNAs were cloned into Sequencing:Article Title: Colorectal cancer risk variants in the 11q13.4 locus are associated with variable POLD3 transcript expression which may promote DNA damage and telomere shortening in colorectal cancer cells Article Snippet: For the Genomics England CRC cohort (N=163), Spearman correlation coefficients were similarly calculated among POLD3 transcripts within genotype-defined groups (heterozygous vs homozygous carriers of the rs577 risk allele). .. POLD3 shRNA target sequences were obtained from Broad institute GPP web portal (Clone ID: TRCN0000233258, TRCN0000233261) and were inserted into shRNA sequences as described here. shRNAs were cloned into other:Article Title: Augment proteasome inhibitor efficacy activates CD8 + T cell-mediated antitumor immunity in breast cancer Article Snippet: |